Inhloso Ukuphenya umthelela kanye nesigaba sengxenye ye-Agkistrodon acutus venom ACTX-8 ekunamatheleni nasekuhlaselweni komdlavuza womlomo wesibeletho iseli iHela.
Vala uhlelo.Izindlela ze-Fibronectin zafakwa epuleti lemithombo engama-96.Amaseli e-Hela adlule ku-0, 2.5, 5, 10, 20, 40 g/ml ye-ACTX-8
Ngemuva kokwelashwa, ukunamathela nge-Fibronectin kutholwe, futhi inani lamangqamuzana anamathele litholwe yi-MTY;Ukutholwa kokuhlasela kwamaseli nokufuduka ngendlela yeseli ye-TransweU
Izinguquko emandleni;Ukubophezela kokuncintisana phakathi kwe-ACTX-8 ne-Fibronectin ne-Hela cell membrane kutholwe nge-flow cytometry.Imiphumela:
Ngemuva kokwelashwa nge-ACTX-8, ukunamathela kwamaseli e-Hela ku-Fibronectin kwehle, futhi ukuhlolwa kwe-transwell cell kwabonisa ukuthi amaseli e-Hela athutha.
Amandla okufuduka nokuhlasela nawo ayancishiswa.Imiphumela ye-cytometry yokugeleza ibonisa ukuthi i-ACTX-8 ingaqhudelana ukuze ibophe isamukeli se-Fibronectin kuseli.
Isikhathi sokuthumela: Feb-18-2023