[Abstract] Injongo: Ukubona umthelela wengxenye I elwa nesimila ye-Agkistrodon acutus venom (AAVC-1) kumsebenzi wokufuduka wamaseli endothelial we-umbilical vein endothelial cells (HUVECs), kanye nokuhlola indlela engaba khona ye-AAVC – Ⅰ inhibiting. i-angiogenesis.Izindlela: Ama-HUVEC akhuliswe ku-vitro futhi aphathwa nge-AAVC - Ⅰ (0, 20, 40, 80 μ G/ml) amaseli aphathwayo afakelwe amahora angama-24.Ukuhlolwa kokuklwebha kanye nokuhlolwa kwegumbi le-chemotactic kwasetshenziswa ukuze kubonwe umphumela we-AAVC – Ⅰ emsebenzini wokufuduka kweseli endothelial;I-RT-PCR kanye ne-Western blot yasetshenziselwa ukuthola izinguquko ze-mRNA namazinga amaprotheni we-P-selectin kanye ne-intercellular adhesion factor (ICAM-1) ngaphambi nangemva kokwelashwa kwezidakamizwa.Imiphumela: Uma kuqhathaniswa nama-HU VECs eqenjini elivamile, amandla okuthutha amaseli ku-AAVC - Ⅰ amaqembu okugxilisa ingqondo ehle afika kumazinga ahlukahlukene, kanye nokuvezwa kwe-P-selectin ne-ICAM-1 mRNA kwehle kakhulu.Isiphetho: I-AAVC – Ⅰ ingase ivimbele umsebenzi wokuthutha wamaseli e-endothelial ngokwehlisa i-mRNA namazinga amaprotheni e-P-selectin ne-ICAM-1
Isikhathi sokuthumela: Nov-29-2022