[Abstract] Inhloso Ukuhlukanisa nokuhlanza amaprotheni e-anticoagulant kusuka ku-Agkistrodon acutus venom eningizimu ye-Anhui kanye nokubona umphumela wawo ovimbelayo kumaseli e-vascular endothelial wemithambo.Izindlela Amaseli e-vascular endothelial womuntu EC-304 akhuliswe ku-vitro.I-CCK-8 colorimetry yasetshenziselwa ukunquma i-cytotoxicity yengxenye yeprotheyini elwa ne-anticoagulant ye-Agkistrodon halys venom kumaseli e-EC-304 akhuliswe ku-vitro.Imakroskopu yokuqhathanisa yesigaba isetshenziselwe ukubheka izinguquko ku-morphology yeseli kanye ne-biochemistry yamaseli e-EC-304 alashwe ngengxenye yeprotheyini ye-anticoagulant ye-Agkistrodon halys venom.Imiphumela Ingxenye ye-anticoagulant protein ye-Agkistrodon halys venom ibe nokuvimbela ukukhula okusobala kumaseli e-EC-304 (P μ Ingxenye ye-anticoagulant protein ye-Agkistrodon halys venom (g/mL) ibe nomthelela oqinile wokuvimbela kumaseli e-EC-304, futhi izinga lokuvinjelwa lenyuka. ngokukhula komthamo; Amaseli e-EC-304 alashwe nge-anticoagulant protein evela ku-Agkistrodon halys venom abonise izinguquko ze-morphological. Ngaphansi kwe-microscope, amaseli ayeyindilinga, inani lamaseli amisiwe lakhula, inani lezigaba zokuhlukaniswa kwamaseli lehla, futhi inani lezingcezu zamaseli endaweni yesiko lenyuke kakhulu.Isiphetho Ingxenye yeprotein ye-anticoagulant ye-Agkistrodon acutus venom evela eningizimu yeSifundazwe sase-Anhui inomphumela oqinile wokuvimbela kumaseli e-EC-304, futhi ukungenisa i-apoptosis yamaseli e-EC-304 kungase kube enye yezindlela zayo.
Isikhathi sokuthumela: Dec-29-2022