[Abstract] Injongo: Ukutadisha umphumela we-Agkistrodon acutus venom suppressor component I (AAVC-I) ekuvimbeleni ukwanda kanye ne-apoptosis yomdlavuza wamaphaphu womuntu A549.Izindlela: Amazinga okuvimbela i-AAVC-I ekugxilweni okuhlukene kumaseli we-A549 we-24h ne-48h alinganiswa ngendlela ye-MTT;I-HE staining kanye ne-Hoechst 33258 fluorescence staining yasetshenziselwa ukubuka i-apoptosis evela ku-morphology;Ukubonakaliswa kwe-bax protein kutholwe yi-immunohistochemistry.Imiphumela: I-MTT ibonise ukuthi i-AAVC-I ingavimbela ukwanda kwamaseli e-A549 ngendlela encike esikhathini futhi encike kumthamo;Ngemuva kokwelashwa kwe-AAVCI amahora angu-24, i-nuclear pyknosis, imizimba ye-nuclear hyperchromatic kanye ne-apoptotic yabonwa ngaphansi kwe-microscope;I-Immunohistochemistry ibonise ukuthi ukuminyana kwe-optical okumaphakathi kwanda ngokukhula kokugxila kwezidakamizwa, okubonisa ukuthi ukuvezwa kwe-bax protein kwakulawulwa ngokuhambisanayo.Isiphetho: Ingxenye I ye-Antitumor I ye-Agkistrodon acutus venom inganqanda umdlavuza wamaphaphu womuntu amaseli angu-A549 futhi yenze i-apoptosis, okungenzeka ihlotshaniswe nokulawulwa okuphezulu kwe-bax expression.
Isikhathi sokuthumela: Jan-16-2023