[Abstract] I-Haemocoagulase Acutus (Halase), i-thermostatic entsha ekuthuthukisweni kwe-halase, iyi-thrombobin esebenza kakhulu efana ne-enzyme (TLE) etholakala ebuhlungwini benyoka ye-Agkistrodon acutus Ukutadisha i-pharmacokinetics ye-Halase, indlela ye-radioisotope enelebula engu-125Ⅰ kanye ne-radioCAssay ehlanganiswe ne-radioCAssay. i-precip-itate isetshenziswe kumagundane.Imiphumela yabonisa ukuthi amanani e-AUC ayehlotshaniswa kahle nemithamo ngaphansi kokubili izindlela ezisetshenzisiwe, futhi amakhoyefiyenti okuxhumana ayengu-0.999 8 kanye no-0.999 0 ngokulandelanayo;ukugxilwa kwezidakamizwa esibindini, ubendeni nasenhliziyweni kufinyelele umvuthwandaba wakho ngemva kwemizuzu emi-5 yokuphatha kuyilapho iningi lamanye ama-organ lifinyelela kukho ngemizuzu engama-30, bese lehla kancane kancane;ngesikhathi ngasinye, ukugcwala kwezidakamizwa esibindini kwakuphakeme kakhulu kunakwezinye izicubu;I-Halase yayikhishwe ngokuphelele futhi ikakhulukazi emchamweni.% I-Thrombin evela ku-Agkistrodon acutus iwuhlobo lwe-thrombin ehlukanisiwe futhi yahlanzwa ebuhlungwini be-Agkistrodon acutus.Iwuhlobo olusha lomuthi we-hemostatic othuthukiswe ithimba lethu I-pharmacokinetics ye-thrombin evela ku-Agkistrodon acutus kumagundane yacwaningwa ngendlela ye-radioisotope engu-125 ebhalwe I-I kanye nemvula ye-trichloroacetic acid kuhlanganiswe nokutholwa komsakazo.Imiphumela yabonisa ukuthi amanani e-AUC akalwa ngezindlela ezimbili ayehlotshaniswa kahle nethamo, futhi ama-coefficients okuxhumana ayengu-0.9998 no-0.9990, ngokulandelana;Okuqukethwe kwezidakamizwa esibindini, ku-spleen nasenhliziyo kwakuyimizuzu emi-5 ephakeme kakhulu ngemva kokuphathwa, kuyilapho ukuthi kwezinye izicubu eziningi kwakuyimizuzu engu-30 ephakeme kakhulu ngemva kokuphathwa, bese kuncipha kancane kancane;Ngesikhathi ngasinye, okuqukethwe kwezidakamizwa ezicutshini zesibindi kwakuphakeme kakhulu kunezinye izicubu;Ngaphezu kwalokho, ukukhishwa kwe-thrombin kwe-Agkistrodon acutus kuphelile, ikakhulukazi ngomchamo.
Isikhathi sokuthumela: Dec-08-2022