[Abstract] [Injongo] Ukulungiselela i-antibody ye-IgY ngokumelene ne-motif evamile ye-metalloproteinase evela ku-Agkistrodon acutus venom, nokufunda umphumela wayo emsebenzini wobuthi benyoka [Izindlela] Ngokuhlaziya ukulandelana okuvamile kwama-metalloproteinase ahlukahlukene kusuka ku-Agkistrodon acutus venom, siklame. futhi kuhlanganiswe ama-antigen peptides e-PT1 ne-PT2 agcinwe kakhulu, ahlobene eduze nomsebenzi we-enzyme;Izikhukhukazi zagonywa ngama-conjugated compounds i-KLH-PT1 ne-KLH-PT2 ukuze zithole amasosha omzimba e-IgY.Isihloko se-IgY kanye nezici zokusabela okuphambene ne-Agkistrodon acutus kanye ne-Agkistrodon acutus venom zacwaningwa yi-ELISA kanye ne-Western blot;Okokugcina, umsebenzi we-IgY ongathathi hlangothi wahlaziywa ngokuhlola ukopha kwe-anti-subcutaneous kumagundane [Imiphumela] Imiphumela ye-ELISA kanye ne-Western blot ibonise ukuthi i-anti-KLH-PT1 kanye ne-anti-Agkistrodon acutus IgY ingase ihlangane ne-Agkistrodon acutus kanye ne-Agkistrodon acutus venom. , futhi lesi sakamuva sasinamandla kakhulu kunesokuqala;I-Anti-KLH-PT2 IgY ayinakho ukusabela okuphambene okusobala ne-Agkistrodon acutus venom kanye ne-Agkistrodon acutus venom in vitro, kodwa inobuthi obuqinile obuqeda amandla ekuhlolweni kokunqanda ukopha ku-vivo, futhi iphakeme kakhulu kunezimbili zangaphambili [Isiphetho] I-IgY amasosha omzimba angakwazi ukusabela ngobuthi benyoka ajikeleza igazi alungiswa ngokuklama ukulandelana okuvamile kwe-antigen peptide ye-metalloproteinase, eyabeka isisekelo sokulungiswa kwe-anti-hemorrhagic enobuthi benyoka ethize, ephumelelayo, ephansi kanye ne-anti-spectrum ebanzi elwa nobuhlungu benyoka.
Isikhathi sokuthumela: Jan-12-2023