I-antibody ye-IgY ngokumelene ne-motif evamile ye-metalloproteinase evela ku-Agkistrodon acutus venom yalungiswa futhi indima yayo ekusebenzeni kwesihlungu senyoka yacwaningwa [Izindlela] Ngokuhlaziya ukulandelana okuvamile kwama-metalloproteinase ahlukahlukene avela ku-Agkistrodon acutus venom, saklama futhi sahlanganisa ama-antigenic nama-peptides PT1 alondolozwe kakhulu. , ezihlobene eduze nemisebenzi yazo ye-enzyme;Izikhukhukazi zagonywa ngama-conjugated complexes i-KLH-PT1 kanye ne-KLH-PT2 ukuze zithole amasosha omzimba e-IgY.Isihloko se-IgY kanye nezici zokusabela eziphambanayo ezinobuthi be-Agkistrodon acutus ne-Agkistrodon brevius zahlolwa kuqala yi-ELISA kanye ne-Western blot;Ekugcineni, umsebenzi we-IgY onciphisa ukopha wahlolwa ukuhlolwa kokuphuma kwegazi okungaphansi kwesikhumba kumagundane [Imiphumela] i-ELISA kanye ne-Western blot yabonisa ukuthi i-anti KLH-PT1 kanye ne-anti Agkistrodon acutus venom IgY zingase zihlangane ne-Agkistrodon acutus venom kanye ne-Agkistrodon brevius venom, kanye ne-latter venom. yayinamandla kakhulu kuneyangaphambili;I-anti KLH-PT2 IgY ibingenakho ukungqubuzana okubalulekile kwe-Agkistrodon acutus kanye ne-Agkistrodon brevius venoms in vitro, kodwa yabonisa ubuthi obuqinile obungaphenduki ekuhlolweni kokunqanda ukopha ku-vivo, futhi yayiphakeme kakhulu kunezimbili zangaphambili [Isiphetho] I-antibody ye-IgY engakwazi. ukusabela okuphambene okunobuthi benyoka obujikelezayo obuhlukahlukene begazi kwalungiselelwa ngokuklama i-antigen peptide eyabelwa ama-metalloproteinase, eyabeka isisekelo sokulungiswa kwe-antibody ethize, esebenza kahle, enobuthi obuphansi kanye ne-anti-spectrum ebanzi yokulwa nobuhlungu benyoka.
Isikhathi sokuthumela: Dec-26-2022