I-Snake Venom thrombin efana ne-enzyme (TLE) iyi-serine protease yomndeni we-trypsin, enokulandelana okulondolozeke kakhulu nge-trypsin.Inomsebenzi we-arginine esterase, ingasebenza ngokuqondile ku-fibrinogen, isungule ukuqhekeka kwebhondi ye-peptide ye-Arg2Gly engxenyeni ethile ye-molecule ye-fibrinogen, ikhulule i-fibrinopeptide A (FPA) noma i-B (FPB. Ama-thrombin ambalwa e-alkaline ahlukaniswa ngekholomu yokushintshisana kwe-cation, kuhlanganiswe i-gel filtration kanye ne-affinity chromatography. I-Affinity chromatography isetshenziswa kakhulu ngenxa yokusebenza kahle kwayo ngokuhlukana nokuthola isampula ephezulu. Kusetshenziswa izinqubo ezihlukene zokuhlanza, futhi imikhiqizo etholiwe nayo ihlukile. U-Guo Chunteng et al. [6, 7] bathole i-thrombin efana nezingxenye I-P3 ne-P4 kusukela ebuhlungwini be-Agkistrodon acutus e-Fujian ngokusebenzisa izinqubo ezihlukene zokuhlanza. Ucwaningo lubonisa ukuthi kokubili kunomsebenzi we-thrombin, kodwa i-EDTA ivimbela kancane ukusebenza kwe-thrombin yengxenye ye-P4 futhi ivimbela ngokuphelele umsebenzi we-coagulase wengxenye P3. Ingxenye P4 ayinayo umsebenzi wesici esicuphayo
Isikhathi sokuthumela: Dec-23-2022