[Abstract] Inhloso Ukunquma izinsalela ze-amino acid ye-N-terminal 15 zamayunithi amabili afanayo e-Agacutin.Amayunithi amabili e-molecule ye-enzyme adinga ukuhlukaniswa ukuze kutholwe iketango le-peptide elilodwa le-subunit futhi alandelwe ngokulandelana.Izindlela I-Agacutin ehlanzwe kakhulu yatholwa ngendlela ye-biochemical.Amasampula eyunithi yeyunithi eyodwa yokuhlonza nokulandelana alungiswe ngokuhlukaniswa kwe-SDS-PAGE electrophoresis, ukutholwa komugqa wokunamathisela we-subunit kanye nobuchwepheshe be-PVDF membrane electrotransfer.Lesi sakamuva sinqume izinsalela ze-amino acid eziyi-15 ku-N-terminal ye-subunit ngendlela ye-Edman yokuwohloka.Imiphumela Ukulandelana kweyunithi enkulu (16 kDa) bekuyi-DCSSGWSSYEEHQYY, kanye nalokho kweyunithi encane (15 kDa) kwaba ngu-DSSGWSSYEGHEYYV.Isiphetho Imiphumela yokulandelana ibonise ukuthi i-Agacutin kwakuyi-thrombin entsha yesihlungu senyoka njenge-enzyme ngokusesha isizindalwazi se-NCBI.
Isikhathi sokuthumela: Dec-22-2022