Injongo: Ukutadisha umphumela wengxenye ye-tumor suppression I (AAVC-I) ekuvimbeleni ukwanda kanye ne-apoptosis yomdlavuza wamaphaphu womuntu amaseli A549.Izindlela: Indlela ye-MTT yasetshenziselwa ukuthola izinga lokuvinjelwa kwe-24h ne-48h lamaseli e-A549 nge-AAVC-I ekugxilweni okuhlukile.
I-HE staining kanye ne-Hoechst33258 fluorescent staining yasetshenziselwa ukubuka i-apoptosis evela ku-morphology;I-Immunohistochemistry yasetshenziselwa ukuthola izinguquko ku-BAX protein expression.Imiphumela: I-MTT ibonise ukuthi i-AAVC-I ingavimbela ukwanda kwamaseli e-A549 ngendlela encike esikhathini nangendlela yomthamo.Ngemuva kokwelashwa kwe-AAVCI ye-24h, ukunqanyulwa kwe-nucleus, i-nuclear hyperstaining kanye nemizimba ye-apoptotic yayibonakala ngaphansi kwe-microscope.I-Immunohistochemistry ibonise ukuthi inani elimaphakathi le-optical density lenyuka ngokukhula kokugxilwa kwezidakamizwa, okubonisa ukuthi ukubonakaliswa kwephrotheni ye-BAX kwakulawulwa ngokufanayo.
Isikhathi sokuthumela: May-11-2023